hordei ( Bgh), 3 but as Bgh does not enter via stomata, the effect, if any, of this closure on the pathogen is not direct.īgh is an obligate biotroph with an ectophytic habit it penetrates only into epidermal cells to form feeding structures (haustoria) that supply the surface mycelia and reproductive structures. Our published study showed that rapid stomatal closure is also a pre-penetration response of barley to the powdery mildew fungus, Blumeria graminis f. 2 showed that bacterial pathogen-associated molecular patterns trigger rapid stomatal closure, and argued that this represents an innate mechanism of resistance against pathogens that enter via stomatal pores. Stomata balance CO 2 uptake with transpiration1 in response to physiological and environmental cues and they also respond to biotic stress including pathogen attack.